Proliferative Activity of Myoepithelial Cells in Normal and Diabetic Parotid Glands Based on Double Immunostaining Labeling

AIM: This study aimed to examine the effect of diabetes mellitus on histology parotid glands and give a scientific overview distribution proliferative activity myoepithelial cells (MECs) encircling both ducts acini in gland normal diabetic mongrel dogs. MATERIALS AND METHODS: Twelve male dogs were used experiment divided into two equal groups, group I, control group, II, with alloxan-induced diabetes. The II injected by fresh preparation single dose 100 mg/kg body weight alloxan monohydrate dissolved physiological saline. Ten days later, blood glucose level was determined using enzymatic colorimetric test; presented at or above 200 mg/dL included experiment. Three months sacrificed from all groups dissected prepared for histological examination double immunohistochemical expression actin proliferating cell nuclear antigen (PCNA). RESULTS: Histological findings H E staining confirmed that parenchyma had glandular atrophy characterized loss structure, acinar degeneration, dilatation duct system presence like structure. Moreover, there predominance fibrous component fat within compartments. Immunohistochemical revealed positive scattered weak mild quantity embracing some intralobular ducts. Expression PCNA actin-positive few reactions small Parotid mild-to-moderate acini, intralobular, interlobular actin- reaction more concentrated surrounding intercalated CONCLUSION: Routine our showed abundant pathological changes parenchymal tissue elements, including acinar, ductal, MECs significant impact saliva production secretion resulting dry mouth. indicated routine regeneration process, whereas might indicate transformation rather than regeneration, especially because no remedial measures taken during this investigation.